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Acta gastroenterol. latinoam ; 33(2): 63-71, 2003.
Article in Spanish | LILACS | ID: lil-420384

ABSTRACT

A simple method, easy to perform during an endoscopic procedure, fast and inexpensive, that allows detecting deficiencies in lactase, sucrase or maltase activities is presented. Briefly, method consists in placing a duodenal biopsy sample in an adequate vial containing lactose, sucrose or maltose solution during a few minutes, and then, adding a few drops of a glucose reactive from commercial origin. Presence of any enzymatic activity is demonstrated when released glucose from any of the disaccharides chosen reacts with the second reactive, turning solution to a red colour. Its utility is discussed and compared with other diagnostic methods.


Subject(s)
Humans , Male , Female , Clinical Enzyme Tests , Disaccharidases/deficiency , Duodenum/enzymology , Intestinal Mucosa/enzymology , Colorimetry , Duodenoscopy , Duodenum/pathology , Intestinal Mucosa/pathology , Lactose/deficiency , Maltose/deficiency , Sucrase/deficiency
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